Clark R B
J Cyclic Nucleotide Res. 1978 Aug;4(4):259-70.
Commercially available adenylylimidodiphosphate, APP(NH)P, appears to be contaminated with a variety of compounds. The effects of unpurified APP(NH)P on adenylate cyclase activity closely resembled the effects of guanylylimidodiphosphate (GPP(NH)P); that is, APP(NH)P alone caused a time-dependent, quasi-irreversible activation, and the stimulation of adenylate cyclase by APP(NH)P in combination with epinephrine was synergistic, eliminating the need for GTP. The GPP(NH)P-like activity of APP(NH)P could be separated from APP(NH)P by purification of the analogue on DEAE cellulose columns or by paper chromatography. The purified APP(NH)P does not appear to interest at the GTP site.
市售的腺苷酰亚胺二磷酸（APP(NH)P）似乎被多种化合物污染。未纯化的APP(NH)P对腺苷酸环化酶活性的影响与鸟苷酰亚胺二磷酸（GPP(NH)P）的影响非常相似；也就是说，单独的APP(NH)P会引起时间依赖性的、近乎不可逆的激活，并且APP(NH)P与肾上腺素联合对腺苷酸环化酶的刺激是协同的，无需GTP。APP(NH)P的GPP(NH)P样活性可以通过在DEAE纤维素柱上纯化该类似物或通过纸色谱法与APP(NH)P分离。纯化后的APP(NH)P似乎不会作用于GTP位点。
