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从两个不同启动子对噬菌体T4 tRNA基因簇进行体外转录。

IN vitro transcription of bacteriophage T4 tRNA gene cluster from two different promoters.

作者信息

Goldfarb A

出版信息

Nucleic Acids Res. 1981 Feb 11;9(3):519-27. doi: 10.1093/nar/9.3.519.

DOI:10.1093/nar/9.3.519
PMID:7220346
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC327219/
Abstract

Analysis of primary transcripts made by Escherichia coli RNA polymerase on T4 DNA containing an intact or partially deleted tRNA gene cluster demonstrates that the T4 tRNA genes are transcribed from two promoters differing in their strength. The stronger (P1) and the weaker (P2) promoters are located at distances of 1 kb and 1.5 kb from the tRNA genes, respectively. Selective initiation of individual transcripts with dinucleotides shows that P1 and P2 promoters contain the sequences TAT and CAC respectively. The two-promoter organisation of the tRNA cluster may reflect two superimposed mechanisms of gene expression in T4-infected bacteria.

摘要

对大肠杆菌RNA聚合酶在含有完整或部分缺失tRNA基因簇的T4 DNA上产生的初级转录本进行分析表明，T4 tRNA基因由两个强度不同的启动子转录。较强的启动子（P1）和较弱的启动子（P2）分别位于距tRNA基因1 kb和1.5 kb处。用二核苷酸对单个转录本进行选择性起始表明，P1和P2启动子分别含有序列TAT和CAC。tRNA簇的双启动子结构可能反映了T4感染细菌中基因表达的两种叠加机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5af/327219/a400cb3f53e9/nar00396-0070-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5af/327219/66beb3a95d3c/nar00396-0069-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5af/327219/a400cb3f53e9/nar00396-0070-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5af/327219/66beb3a95d3c/nar00396-0069-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5af/327219/a400cb3f53e9/nar00396-0070-a.jpg

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引用本文的文献

1
Control of promoter utilization by bacteriophage T4-induced modification of RNA polymerase alpha subunit.噬菌体T4诱导的RNA聚合酶α亚基修饰对启动子利用的控制
Nucleic Acids Res. 1981 Oct 10;9(19):4863-78. doi: 10.1093/nar/9.19.4863.
2
Mapping of in vitro transcription units and identification of primary transcripts of the D region of bacteriophage T4.
Nucleic Acids Res. 1981 Jun 25;9(12):2791-800. doi: 10.1093/nar/9.12.2791.
3
Changes in the promoter range of RNA polymerase resulting from bacteriophage T4-induced modification of core enzyme.噬菌体T4诱导的核心酶修饰导致RNA聚合酶启动子范围的变化。

本文引用的文献

1
Transcriptional control of two gene subclusters in the tRNA operon of bacteriophage T4.噬菌体T4的tRNA操纵子中两个基因亚簇的转录调控。
Nature. 1980 Jul 24;286(5771):418-20. doi: 10.1038/286418a0.
2
Bacteriophage T4 gene transcription studied by hybridization to cloned restriction fragments.通过与克隆的限制性片段杂交研究噬菌体T4基因转录
J Mol Biol. 1980 Apr 15;138(3):423-45. doi: 10.1016/s0022-2836(80)80011-8.
3
DNA sequence of a T4 transfer RNA gene cluster.T4转移RNA基因簇的DNA序列。
Proc Natl Acad Sci U S A. 1981 Jun;78(6):3454-8. doi: 10.1073/pnas.78.6.3454.
4
T4-induced antipolarity: temporal heterogeneity in response of early transcription units.T4诱导的反极性：早期转录单元反应中的时间异质性。
J Virol. 1984 Apr;50(1):191-201. doi: 10.1128/JVI.50.1.191-201.1984.
5
A physical map of bacteriophage T4 including the positions of strong promoters and terminators recognized in vitro.噬菌体T4的物理图谱，包括体外识别的强启动子和终止子的位置。
Mol Gen Genet. 1984;194(1-2):232-40. doi: 10.1007/BF00383522.
J Mol Biol. 1980 May 25;139(3):377-91. doi: 10.1016/0022-2836(80)90136-9.
4
The cloning of a T4 transfer RNA gene cluster.T4转移RNA基因簇的克隆
J Mol Biol. 1980 Feb 25;137(2):213-34. doi: 10.1016/0022-2836(80)90326-5.
5
A restriction map of the T4 transfer RNA gene cluster.
J Mol Biol. 1980 Feb 25;137(2):191-211. doi: 10.1016/0022-2836(80)90325-3.
6
A new method of large scale preparation of highly purified DNA-dependent RNA-polymerase from E. coli.一种从大肠杆菌大规模制备高纯度依赖DNA的RNA聚合酶的新方法。
Hoppe Seylers Z Physiol Chem. 1970 Feb;351(2):221-4. doi: 10.1515/bchm2.1970.351.1.221.
7
Bacteriophage T4 gene expression. Evidence for two classes of prereplicative cistrons.噬菌体T4基因表达。两类复制前顺反子的证据。
J Biol Chem. 1973 Aug 10;248(15):5502-11.
8
Bacteriophage T4 transfer RNA. 3. Clustering of the genes for the T4 transfer RNA's.噬菌体T4转移RNA。3. T4转移RNA基因的聚类。
J Mol Biol. 1972 Nov 28;71(3):547-56. doi: 10.1016/s0022-2836(72)80022-6.
9
Initiation and transcription of a set of transfer ribonucleic acid genes in vitro.一组转移核糖核酸基因在体外的起始与转录
J Biol Chem. 1975 Feb 10;250(3):934-8.
10
Isolation and characterization of conditional lethal mutations in the mot gene of bacteriophage T4.
J Mol Biol. 1978 Dec 15;126(3):551-70. doi: 10.1016/0022-2836(78)90058-x.