皮脂成分：薄层色谱法与红外反射光谱法的比较

Sebum composition: a comparison of thin-layer chromatography and infrared reflectance spectroscopy.

作者信息

Lewis S J, Shalita A R, Lee W L

出版信息

J Invest Dermatol. 1978 Dec;71(6):370-1. doi: 10.1111/1523-1747.ep12556760.

Abstract

Thin-layer chromatography and infrared reflectance spectroscopy were compared as methods for measuring sebum composition. Solutions of varying known concentrations of free fatty acids and triglycerides were analyzed by both methods. Skin surface lipid samples were similarly analyzed. Infrared spectroscopy produced more variable data than thin-layer chromatography with photodensitometry. This was particularly true at low concentrations of free fatty acids. It is concluded that thin-layer chromatography with photodensitometry is a more accurate method for the measurement of sebum composition.

摘要

比较了薄层色谱法和红外反射光谱法作为测量皮脂成分的方法。用这两种方法分析了不同已知浓度的游离脂肪酸和甘油三酯溶液。对皮肤表面脂质样本也进行了类似分析。与采用光密度测定法的薄层色谱法相比，红外光谱法产生的数据变异性更大。在游离脂肪酸浓度较低时尤其如此。结论是，采用光密度测定法的薄层色谱法是测量皮脂成分更准确的方法。