Daily O P, Gillmore J D
Undersea Biomed Res. 1981 Mar;8(1):23-31.
The effect of hyperbaric stress on the composition of fecal microflora was investigated in the mouse. Mice were exposed to 35 ATA with normoxic PO2 concentration in a helium atmosphere at 35 degrees C to prevent body heat loss or to 1 ATA oxygen-helium at 25 degrees C. Freshly discharged fecal pellets were cultured under strict anaerobic conditions, replica plated, and then cultured both aerobically and anaerobically. From experiment to experiment there was considerable variation in the total number of cultured bacteria. By replica plating, however, a consistent reduction in the relative numbers of obligate anaerobes was demonstrated, from a mean of 71% to 44%, in the pressurized mice. Bacteroids distasonis and B. multiacidus were the obligately anaerobic species most frequent identified from mice exposed at 35 and 1 ATA. Major changes in species distribution were not encountered.
在小鼠中研究了高压应激对粪便微生物群组成的影响。将小鼠置于35℃的氦气环境中，暴露于35ATA且常氧PO2浓度下以防止体温流失，或将其置于25℃的1ATA氧氦环境中。将新鲜排出的粪便颗粒在严格厌氧条件下培养，进行影印接种，然后分别在需氧和厌氧条件下培养。每次实验中，培养细菌的总数存在相当大的差异。然而，通过影印接种证明，在受压小鼠中，专性厌氧菌的相对数量持续减少，从平均71%降至44%。狄氏拟杆菌和多酸拟杆菌是在35ATA和1ATA暴露小鼠中最常鉴定出的专性厌氧物种。未发现物种分布有重大变化。
