Saxén L, Lehtonen E
J Embryol Exp Morphol. 1978 Oct;47:97-109.
The kinetics of kidney tubule induction were examined in transfilter experiments by varying the time of transfilter apposition, the porosity and pore size of the filters, and the culture conditions. Transfilter contact between the interacting cells is established within an hour when cytoplasmic processes emerge through the interposed filter; then a further 16-24 h are needed for completion of induction. This lag is a function of thickness and pore size of the filter, and is not reduced by precultivation of the inductor on the filter. Material that accumulates on the far side of the filter during such cultivation displays no morphogenetic activity. The intensity of the mesenchymal response was roughly quantified and shown to be a function of pore size, pore density and duration of transfilter contact.
通过改变滤器并置时间、滤器的孔隙率和孔径以及培养条件，在滤器间实验中研究了肾小管诱导的动力学。当细胞质突起穿过插入的滤器时，相互作用的细胞之间在一小时内建立滤器间接触；然后完成诱导还需要16 - 24小时。这种延迟是滤器厚度和孔径的函数，并且不会因诱导物在滤器上的预培养而缩短。在这种培养过程中积聚在滤器另一侧的物质没有形态发生活性。间充质反应的强度大致定量，并显示为孔径、孔密度和滤器间接触持续时间的函数。
