Escalona-Zapata J, Alvarez Fernandez E, Llorca Escuin F
Virchows Arch A Pathol Anat Histol. 1981;391(2):165-75. doi: 10.1007/BF00437594.
Dermatofibrosarcoma protuberans has been considered to be of fibrohistiocytic or fibroblastic origin. The purpose of this paper is to identify the original cell strain from which this neoplasm derives, using tissue culture and electron microscopic methods. Thirteen cases of DFSP characterised by clinical, topographical, histological and behavioral criteria were explanted. The emigrating cells were bipolar with two opposed processed and showed a radial arrangement in respect to the explants. After the second week the distal processes tended to curve back towards the cell body forming flame-like structures. This cell morphology and cellular orientation persisted during the whole life of the culture. Electron microscopy was performed in three cases; the newly grown cells maintained an electron microscopic picture similar to that found in the original tumors. This pattern of behaviour is characteristic of fibroblastic tumors and has been found in explants of normal fibroblasts, of fibromatosis and of fibrosarcomas used as controls. On this basis, we believe that DFSP is a fibrosarcoma of the skin of low grade malignancy.
隆突性皮肤纤维肉瘤一直被认为起源于纤维组织细胞或成纤维细胞。本文旨在通过组织培养和电子显微镜方法确定该肿瘤起源的原始细胞株。选取了13例符合临床、部位、组织学和生物学行为标准的隆突性皮肤纤维肉瘤病例进行外植培养。迁移出的细胞呈双极形，有两个相对的突起，并相对于外植体呈放射状排列。第二周后，远端突起往往向细胞体弯曲，形成火焰状结构。这种细胞形态和细胞取向在培养的整个过程中持续存在。对3例进行了电子显微镜检查；新生长的细胞保持了与原始肿瘤相似的电子显微镜图像。这种行为模式是成纤维细胞性肿瘤的特征，在用作对照的正常成纤维细胞、纤维瘤病和纤维肉瘤的外植体中也有发现。基于此，我们认为隆突性皮肤纤维肉瘤是一种低级别恶性的皮肤纤维肉瘤。
