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固定化微生物进行的甾体转化。VI. 固定化状态下不同微生物甾体-1-脱氢酶的逆反应

Steroid transformation with immobilized microorganisms. VI. The reverse reaction of steroid-1-dehydrogenases from different micoorganisms in immobilized state.

作者信息

Atrat P, Groh H

出版信息

Z Allg Mikrobiol. 1981;21(1):3-6. doi: 10.1002/jobm.3630210102.

DOI:10.1002/jobm.3630210102
PMID:7222747
Abstract

Whole cells of Nocardia erythropolis, N. opaca and Mycobacterium phlei containing 4-en-3-oxosteroid: (acceptor)-1-en-oxidoreductase activities were immobilized by adsorption on DEAE-cellulose and silica and by entrapment in polyacrylamide gel. The obtained biocatalysts were used in an anaerobic continuous column process to transform 1,4-dien-3-oxo-steroids into 4-en-3-oxo- steroids. The half life of steroid-1(2)-reductase activity of the N. erythropolis was found to be up to 15 days. The DEAE-cellulose-adsorbed cells showed a slightly higher relative activity than free cells. The influence of substrate concentration and the action of dimethylformamide were also investigated.

摘要

包含4-烯-3-氧代甾体：（受体）-1-烯氧化还原酶活性的红斑诺卡氏菌、不透明诺卡氏菌和草分枝杆菌的全细胞，通过吸附在DEAE-纤维素和硅胶上以及包埋在聚丙烯酰胺凝胶中进行固定化。将所得生物催化剂用于厌氧连续柱过程，以将1,4-二烯-3-氧代甾体转化为4-烯-3-氧代甾体。发现红斑诺卡氏菌的甾体-1(2)-还原酶活性的半衰期长达15天。DEAE-纤维素吸附的细胞显示出比游离细胞略高的相对活性。还研究了底物浓度的影响和二甲基甲酰胺的作用。

相似文献

1
Steroid transformation with immobilized microorganisms. VI. The reverse reaction of steroid-1-dehydrogenases from different micoorganisms in immobilized state.固定化微生物进行的甾体转化。VI. 固定化状态下不同微生物甾体-1-脱氢酶的逆反应
Z Allg Mikrobiol. 1981;21(1):3-6. doi: 10.1002/jobm.3630210102.
2
[Steroid-transforming enzymes from microorganisms. XII. Induction characteristics of the 4-en-3-oxosteroid: (acceptor)-1-en-oxidoreductase in Nocardia opaca].
Z Allg Mikrobiol. 1979;19(10):731-9.
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[Steroid-transforming enzymes from microorganisms. XI. Reversibility of the dehydrogenation reaction of the steroid-1-dehydrogenase from Nocardia opaca].[微生物甾体转化酶。十一。不透明诺卡氏菌甾体-1-脱氢酶脱氢反应的可逆性]
Z Allg Mikrobiol. 1979;19(10):727-30.
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[Steroid transforming enzymes from microorganisms. V. Purification of a 4-en-3-oxosteroid: (acceptor)-1-en-oxidoreductase from Nocardia opaca and characterization of the prosthetic group].
Z Allg Mikrobiol. 1978;18(3):189-96. doi: 10.1002/jobm.3630180306.
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[Steroid-transforming enzymes from microorganisms. IX. Affinity chromatographic preparation and studies of the apoenzyme from a 4-en-3-oxosteroid: (acceptor)-1-en-oxidoreductase from Nocardia opaca].
Z Allg Mikrobiol. 1979;19(5):315-8. doi: 10.1002/jobm.3630190502.
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[Steroid transformation with immobilized microorganisms. III. Degradation of the side chain of cholesterol derivatives with immobilized Mycobaterium phlei and M. smegmatis cells].[固定化微生物对类固醇的转化。III. 用固定化草分枝杆菌和耻垢分枝杆菌细胞降解胆固醇衍生物的侧链]
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[Steroid conjugating enzyme from microorganisms. VI. Purification of 4-en-o-oxosteroid: (acceptor)-1-en-oxidoreductase from Nocardia opaca by affinity chromatography].[来自微生物的类固醇结合酶。VI. 通过亲和色谱法从不透明诺卡氏菌中纯化4-烯-o-氧代类固醇：（受体）-1-烯氧化还原酶]
J Steroid Biochem. 1977 Sep;8(9):1017-24. doi: 10.1016/0022-4731(77)90201-1.
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[Steroid converting enzymes from microorganisms. VIII. Effect of the organic solvent specific hydrophobicity of the binding on the affinity-matrix- affinity chromatography of a 4-en-3-oxo-steroid: (Acceptor)-1-en-oxidoreductase from Nocardia opaca].[来自微生物的类固醇转化酶。VIII. 结合的有机溶剂特定疏水性对4-烯-3-氧代类固醇：（受体）-1-烯氧化还原酶（来自不透明诺卡氏菌）亲和基质亲和色谱的影响]
J Steroid Biochem. 1978 Jul;9(7):691-4. doi: 10.1016/0022-4731(78)90183-8.
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[Steroid transforming enzymes in microorganisms. XIII. Relationship between steroid structure and induction of 4-ene-3-oxosteroid: (acceptor)-1-ene-oxidoreductase in Nocardia opaca].[微生物中的类固醇转化酶。 XIII. 诺卡氏菌中类固醇结构与4-烯-3-氧代类固醇：（受体）-1-烯氧化还原酶诱导之间的关系]
Z Allg Mikrobiol. 1980;20(1):23-32. doi: 10.1002/jobm.19800200104.
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[Steroid metabolizing enzymes from microorganisms. VII. The nature of the biospecific binding to an affinity matrix-affinity chromatography of 4-en-3-oxo-steroid: (acceptor)-1-en-oxidoreductase from Nocardia opaca].[来自微生物的类固醇代谢酶。VII. 与亲和基质的生物特异性结合的性质——不透明诺卡氏菌4-烯-3-氧代类固醇：（受体）-1-烯氧化还原酶的亲和色谱法]
J Steroid Biochem. 1978 Jun;9(6):579-82. doi: 10.1016/0022-4731(78)90125-5.

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