Viring S
Acta Vet Scand. 1980;21(4):587-97. doi: 10.1186/BF03546846.
Twenty-four gilts were inseminated pair-wise with live or dead spermatozoa from the same ejaculate. The insemination dose was 100 ml undiluted semen containing, on average, 19×10 spermatozoa. The gilts were slaughtered 1, 2, 6 and 12 h after insemination. The numbers of spermatozoa were counted in the uterus, uterotubal junction and in four equally long segments of the oviduct, called I–IV, with a haemocytometer. IV was adjacent to the uterotubal junction. The numbers of spermatozoa recovered in the uterus diminished significantly during the first 12 h after insemination. From gilts inseminated with live spermatozoa more spermatozoa were recovered in the uterotubal junction than from gilts inseminated with dead spermatozoa. Two h after insemination spermatozoa were recovered in all oviducts. Significantly more live than dead spermatozoa were recovered in Segments III and IV of the oviduct, regardless of time. In gilts inseminated with live spermatozoa the sperm count in Segment I varied significantly with time, being hiigest 2 h after insemination. At 6 and 12 h there were no distinct differences in the distribution of live spermatozoa between the various oviduct segments. The numbers of spermatozoa recovered in the oviduct were at these times apparently related to the sperm depots in the uterotubal junction.
24头后备母猪两两配对，用来自同一射精量的活精子或死精子进行授精。授精剂量为100毫升未稀释精液，平均含有19×10⁶个精子。在授精后1、2、6和12小时屠宰这些后备母猪。用血细胞计数器对子宫、子宫输卵管连接处以及输卵管四个等长段（称为I - IV段）中的精子数量进行计数。IV段与子宫输卵管连接处相邻。授精后前12小时内，子宫中回收的精子数量显著减少。与用死精子授精的后备母猪相比，用活精子授精的后备母猪在子宫输卵管连接处回收的精子更多。授精后2小时，在所有输卵管中都回收了精子。无论时间如何，在输卵管的III段和IV段中回收的活精子明显多于死精子。在用活精子授精的后备母猪中，I段的精子计数随时间变化显著，在授精后2小时最高。在6小时和12小时，各输卵管段之间活精子的分布没有明显差异。此时在输卵管中回收的精子数量显然与子宫输卵管连接处的精子储存有关。
