Garrison C P, Deegan M J, Conn R B
Am J Clin Pathol. 1981 Apr;75(4):553-7. doi: 10.1093/ajcp/75.4.553.
Sera from three patients initially evaluated by cellulose acetate electrophoresis demonstrated apparent application "artifacts." On agarose gel electrophoresis, each had a definite paraprotein "spike." The immunoglobulin classes of the paraproteins were determined by immunoelectrophoresis, with and without reduction by dithiothreitol. The immunoglobulin class was confirmed by immunofixation electrophoresis on agarose gel. All three abnormal immunoglobulins were IgM-kappa-type paraproteins. Agarose electrophoresis with immunofixation was superior in the identification of these paraproteins compared with the standard method of cellulose acetate electrophoresis coupled with immunoelectrophoresis.
最初通过醋酸纤维素电泳进行评估的三名患者的血清显示出明显的应用“假象”。在琼脂糖凝胶电泳中，每个人都有一个明确的副蛋白“峰”。通过免疫电泳确定副蛋白的免疫球蛋白类别，使用二硫苏糖醇进行还原或不还原。通过琼脂糖凝胶上的免疫固定电泳确认免疫球蛋白类别。所有三种异常免疫球蛋白均为IgM-κ型副蛋白。与醋酸纤维素电泳结合免疫电泳的标准方法相比，琼脂糖免疫固定电泳在这些副蛋白的鉴定方面更具优势。
