Sukroongreung S, Nilakul C, Sirinanunta S
Am J Med Technol. 1981 Apr;47(4):237-9.
Two-hundred-fifty-five staphylococcal isolates (clinical) were tested for both free coagulase (CGase) and thermostable nuclease (TNase) production. Two-hundred-ten of the 255 isolates were identified as Staphylococcus aureus when both strongly positive TNase and CGase reactions (human and rabbit plasma) were observed with these strains. Forty-five of the 255 isolates were identified as Staphylococcus species, other than S. aureus when either weakly positive TNase and CGase (rabbit plasma) reactions or negative TNase and CGase (rabbit and human plasma) reactions were observed. This experiment suggest that the identification of staphylococcal isolates as S. aureus can be based upon demonstration of both strongly positive TNase and CGase reactions.
对255株临床分离的葡萄球菌进行了游离凝固酶(CGase)和耐热核酸酶(TNase)产生情况的检测。在这255株分离菌中,当观察到这些菌株的TNase和CGase反应(人血浆和兔血浆)均呈强阳性时,其中210株被鉴定为金黄色葡萄球菌。当观察到TNase和CGase反应(兔血浆)呈弱阳性或TNase和CGase反应(兔血浆和人血浆)呈阴性时,255株分离菌中的45株被鉴定为除金黄色葡萄球菌之外的葡萄球菌属。该实验表明,葡萄球菌分离株鉴定为金黄色葡萄球菌可基于TNase和CGase反应均呈强阳性。