Lung B, Cunha G R
Anat Rec. 1981 Jan;199(1):73-88. doi: 10.1002/ar.1091990108.
Seminal vesicles (SV) and coagulating glands (CG) from neonatal mice 1- to 7-days old were observed in whole mount preparations. Untreated, normal SV developed elaborate epithelial branches beginning on day 3 with secondary branches appearing on day 6. Castration (C), estradiol treatment (E), and castration combined with estradiol treatment (C + E) inhibited the morphogenesis of the epithelial branches. Untreated CG formed solid epithelial stalks that developed lateral epithelial buds on day 3 which attained a complex morphological pattern by day 7. Treatment groups (C, E and C + E) displayed a pattern of retarded growth with few epithelial buds appearing even at day 7. The effects of castration on both SV and CG were reversed by the addition of testosterone. Short term in vitro culture of 1-day-old SV and CG glands in control medium or medium supplemented with estradiol did not exhibit visible growth. Culture of SV and CG glands with testosterone or a piece of testis showed pronounced development.
对1至7日龄新生小鼠的精囊（SV）和凝固腺（CG）进行了整装制片观察。未经处理的正常精囊在第3天开始形成复杂的上皮分支，第6天出现二级分支。去势（C）、雌二醇处理（E）以及去势联合雌二醇处理（C + E）均抑制了上皮分支的形态发生。未经处理的凝固腺形成实心的上皮茎，在第3天长出外侧上皮芽，并在第7天形成复杂的形态模式。处理组（C、E和C + E）呈现出生长迟缓的模式，即使在第7天也很少有上皮芽出现。添加睾酮可逆转去势对精囊和凝固腺的影响。在对照培养基或添加雌二醇的培养基中对1日龄精囊和凝固腺进行短期体外培养，未观察到明显生长。用睾酮或一块睾丸培养精囊和凝固腺则显示出明显的发育。
