Mangold B L, Knopf P M
J Parasitol. 1978 Oct;64(5):813-21.
The recovery of schistosomula from lungs of normal and resistant Sprague-Dawley and Fischer rats was analyzed by varying the assay conditions. The effects of incubation medium, incubation time, and perfusion procedure on yields of schistosomula were significant and additive. Optimal assay conditions were established to recover schistosomula by the lung recovery assay. Differences were observed in the number of schistosomula recovered from lungs of normal and resistant rats; the assay was performed as a function of time after challenge infection. Differences in schistosomula recovered were significant on days 3 to 5 (Fischer) and days 3 to 6 (Sprague-Dawley); afterwards, no differences were observed in recoveries of schistosomula from lungs. Interpretations of these findings are disscused.
通过改变检测条件，分析了正常和抗性的斯普拉格-道利大鼠及费希尔大鼠肺内血吸虫童虫的回收率。孵育培养基、孵育时间和灌注程序对血吸虫童虫产量的影响显著且具有累加性。通过肺回收试验确定了回收血吸虫童虫的最佳检测条件。观察到从正常大鼠和抗性大鼠肺内回收的血吸虫童虫数量存在差异；该试验是在攻击感染后的不同时间进行的。在第3至5天（费希尔大鼠）和第3至6天（斯普拉格-道利大鼠），回收的血吸虫童虫存在显著差异；之后，未观察到肺内血吸虫童虫回收率的差异。对这些发现进行了讨论。
