Serrentino R, Gervasi P G
Boll Soc Ital Biol Sper. 1980 Nov 30;56(22):2393-7.
In the present paper, a simple photometric assay of microsomal epoxide hydrolase activity using the 4-NBP test is described. The outline of this method is the reaction of 4-NBP with the unreacted oxide extracted from incubation medium in separated ethyl-acetate layer, followed by measurement of absorbancy at 560 nm. The usefulness of this assay can be in application to all epoxides enough alkylating and it can be used for routine analyses which don't request either particular instruments or suitable standards of synthesis.
本文描述了一种使用4-硝基苯硼酸（4-NBP）试验对微粒体环氧化物水解酶活性进行简单光度测定的方法。该方法的要点是4-NBP与从孵育介质中提取到分离的乙酸乙酯层中的未反应氧化物发生反应，随后在560nm处测量吸光度。该测定法的实用性在于可应用于所有具有足够烷基化能力的环氧化物，并且可用于既不需要特殊仪器也不需要合适合成标准品的常规分析。
