Nishi A, Masumoto K, Asamizu T, Katoh T, Kinpara H
Biochim Biophys Acta. 1981 Mar 18;673(3):243-52.
Hexosamine metabolism in relation to the spherule-wall synthesis in Physarum polycephalum was studied by the incorporation of labeled sugar into the wall and intermediary compounds in the biosynthesis of wall polysaccharide. The incorporation of [14C]galactosamine into the wall material occurred after a lag period of about 10 h in an induction medium. Nucleotides and sugar phosphates in the acid-soluble fraction of spherulating plasmodia were analyzed by column chromatography on Dowex 1-X8 (formate). The primary labeled products formed in the spherulating plasmodia after incubation with [14C]galactosamine were galactosamine 1-phosphate, UDP-galactosamine, N-acetylhexosamine 6-phosphate and UDP-N-acetylhexosamine. Spherulation was insensitive to polyoxin D, while it was completely blocked by cycloheximide. The activity of galactokinase and galactose-1-phosphate uridyltransferase increased 4--5-fold during the spherulation.
通过将标记糖掺入多头绒泡菌细胞壁及细胞壁多糖生物合成中的中间化合物，研究了己糖胺代谢与该菌细胞壁合成的关系。在诱导培养基中，[14C]半乳糖胺掺入壁物质的过程在约10小时的延迟期后发生。采用Dowex 1-X8（甲酸盐）柱色谱法分析了正在形成孢子的原质团酸溶性部分中的核苷酸和糖磷酸酯。用[14C]半乳糖胺孵育后，正在形成孢子的原质团中形成的主要标记产物是半乳糖胺1-磷酸、UDP-半乳糖胺、N-乙酰己糖胺6-磷酸和UDP-N-乙酰己糖胺。孢子形成对多氧霉素D不敏感，而环己酰亚胺可完全阻断其过程。在孢子形成过程中，半乳糖激酶和半乳糖-1-磷酸尿苷酰转移酶的活性增加了4至5倍。
