Ataullakhanov F I, Pichugin A V
Biofizika. 1981 Jan-Feb;26(1):86-8.
Modification of luciferine-luciferase method of determining ATP concentration in erythrocytes is proposed which takes 1.5-2 min from the moment of sample selection to obtaining the result. The method is based on the phenomenon that inhibition of luciferase reaction by an increase of ionic strength (above 200 m-ekv) results in the reaction change. Luminescence intensity rapidly reaches some level slightly changing in time. Successive repeated additions of ATP increase this level in proportion which makes possible to introduce the calibrating ATP solution into the sample under measurement after the intensity has reached the constant value. The method allows measurings of ATP concentration in aqueous hemolysates of erythrocytes or integral blood without protein precipitation.
提出了一种对用于测定红细胞中ATP浓度的荧光素-荧光素酶法的改进方法，从选取样品到获得结果只需1.5至2分钟。该方法基于这样一种现象：离子强度增加（超过200 m-ekv）对荧光素酶反应的抑制会导致反应发生变化。发光强度会迅速达到某个随时间略有变化的水平。连续多次添加ATP会使该水平成比例增加，这使得在强度达到恒定值后，可将校准ATP溶液引入被测样品中。该方法能够在不进行蛋白质沉淀的情况下，测定红细胞水溶血产物或全血中的ATP浓度。
