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来自海拉细胞的[35S] - 甲硫氨酸标记的多肽。一些主要多肽的坐标和百分比。

[35S]-methionine labelled polypeptides from HELA cells. Coordinates and percentage of some major polypeptides.

作者信息

Bravo R, Bellatin J, Celis J E

出版信息

Cell Biol Int Rep. 1981 Jan;5(1):93-6. doi: 10.1016/0309-1651(81)90162-4.

DOI:10.1016/0309-1651(81)90162-4
PMID:7226243
Abstract

A total of 1169 [35S]-methionine labelled polypeptides (855-acidic (IEF) and 314 basic (NEPHGE) from 100 asynchronous human HeLa cells have been separated using high resolution two dimensional gel electrophoresis. Beside numbering these spots, we give for the major polypeptides their coordinates (M.W. and relative mobility respect to beta-actin (IEF) or NEPHGE), and percentage of total protein label. A few major polypeptides have been identified.

摘要

使用高分辨率二维凝胶电泳从100个异步人类HeLa细胞中分离出总共1169种[35S] - 甲硫氨酸标记的多肽（855种酸性（IEF）和314种碱性（NEPHGE））。除了对这些斑点进行编号外，我们还给出了主要多肽的坐标（分子量以及相对于β-肌动蛋白（IEF）或NEPHGE的相对迁移率）和总蛋白质标记的百分比。已经鉴定出一些主要多肽。

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