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Improved enzymatic assay for methotrexate: shape of standard curve, stability of reagents, sensitivity.

作者信息

Scheufler E

出版信息

Clin Chim Acta. 1981 Mar 19;111(1):113-6. doi: 10.1016/0009-8981(81)90429-0.

DOI:10.1016/0009-8981(81)90429-0
PMID:7226540
Abstract

An improved enzymatic assay for methotrexate (MXT) is presented. A linear calibration curve for MTX could only be obtained, when a pre-incubation period of MTX with the enzyme preceded the measurement of dihydrofolate reductase (DHFR) activity. Stability for 24 h was achieved by addition of NaN3 to a working solution containing DHFR, albumin, NADPH, and buffer. The detection limit of a rapid assay was 4 X 10(-9) mol/l MTX in plasma. A second but slow assay with increased detection limit (1 X 10(-9) mol/l) is reported.

摘要

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引用本文的文献

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