一种用于人血清卵磷脂胆固醇酰基转移酶的不依赖脂蛋白的检测方法。

A lipoprotein independent assay for human serum lecithin-cholesterol acyltransferase.

作者信息

Morin R J, Piran U

出版信息

Clin Chim Acta. 1981 Apr 9;111(2-3):211-8. doi: 10.1016/0009-8981(81)90188-1.

Abstract

A method has been developed for estimation of human serum lecithin-cholesterol acyltransferase free of interference by endogenous lipoproteins. Precipitation of serum low and very low density lipoproteins by sodium phosphotungstate and magnesium chloride results in complete recovery of lecithin-cholesterol acyltransferase activity in the supernatant. One microliter of the supernatant can be accurately assayed with a highly efficient substrate containing phosphatidylcholine-cholesterol vesicles and apo-high density lipoproteins (HDL), with no interference from endogenous HDL or residual precipitation reagents. Serum levels of the enzyme were found to be reduced in patients with parenchymal liver disease, renal disease, gastrointestinal tumors and anemias.

摘要

已开发出一种用于估算人血清卵磷脂胆固醇酰基转移酶的方法，该方法不受内源性脂蛋白的干扰。用磷钨酸钠和氯化镁沉淀血清中的低密度和极低密度脂蛋白，可使上清液中的卵磷脂胆固醇酰基转移酶活性完全恢复。一微升上清液可用含有磷脂酰胆碱 - 胆固醇囊泡和载脂蛋白高密度脂蛋白（HDL）的高效底物进行准确测定，不受内源性HDL或残留沉淀试剂的干扰。发现实质性肝病、肾病、胃肠道肿瘤和贫血患者的血清酶水平降低。