Cisson C M, McQuarrie C H, Sketelj J, McNamee M G, Wilson B W
Dev Neurosci. 1981;4(2):157-64. doi: 10.1159/000112752.
Multiple molecular forms of acetylcholinesterase (AChE) from embryonic chick pectoral muscle were examined by sucrose density sedimentation. Embryonic muscle in ovo possessed three molecular forms with apparent sedimentation coefficient of 7S, 11S, and 20S. The proportion of the 20S form increased 4-fold between days 11 and 18 in ovo and there was a decrease in the proportion of the 7S and 11S forms. Cultured embryonic muscle contained only 7S and 11S AChE despite the presence of the 20S form in the tissue from which the cultures were derived. Brief treatment with diisopropylphosphorofluoridate (DFP) completely inhibited the enzyme activity; the newly synthesized AChE first appeared as a 7S form. Polyacrylamide gel electrophoresis of the peak fractions from the sucrose gradients revealed that the 7S AChE was composed of two electrophoretic forms and gave evidence that the 20S AChE was too large to enter the running gel. The results are consistent with the ideas that the distribution of AChE molecular forms is regulated during embryonic development and that AChE is first synthesized as a low molecular weight form following inhibition with organophosphate compounds.
利用蔗糖密度沉降法对来自胚胎期鸡胸肌的多种分子形式的乙酰胆碱酯酶（AChE）进行了检测。胚胎期鸡胚内的肌肉具有三种分子形式，其表观沉降系数分别为7S、11S和20S。在胚胎发育的第11天到第18天之间，20S形式的比例增加了4倍，而7S和11S形式的比例则有所下降。尽管培养胚胎肌肉所源自的组织中存在20S形式，但培养的胚胎肌肉中仅含有7S和11S的AChE。用二异丙基氟磷酸酯（DFP）进行短暂处理可完全抑制酶活性；新合成的AChE最初以7S形式出现。对蔗糖梯度中的峰部分进行聚丙烯酰胺凝胶电泳显示，7S AChE由两种电泳形式组成，并且有证据表明20S AChE太大而无法进入运行凝胶。这些结果与以下观点一致，即AChE分子形式的分布在胚胎发育过程中受到调节，并且AChE在受到有机磷化合物抑制后首先以低分子量形式合成。
