变形链球菌中溶血素产生的检测与特性分析
Detection and characterization of hemolysin production in Streptococcus mutans.
作者信息
Woltjes J, Legdeur-Velthuis H, de Graaff J
出版信息
Infect Immun. 1981 Mar;31(3):850-5. doi: 10.1128/iai.31.3.850-855.1981.
Beta-hemolytic strains of Streptococcus mutans were identified and physiological reactions and hemolysin production were studied. Strict anaerobic conditions were shown to be indispensable for the expression of beta-hemolysis on blood agar plates; hemolysis was only observed after growth in an atmosphere with an oxygen content of 10 ppm or lower. The occurrence of beta-hemolytic strains was found not to be restricted to one biotype of S. mutans. Two hemolytic activities were detected in liquid cultures. Oxygen-labile activity was detected in prereduced broth cultures and was shown to be unstable and indetectable in supernatants. Solubilization of oxygen-labile hemolysin was achieved by the addition of Tween 80 to the cultures. Oxygen-stable activity was detected in supernatants of cell suspensions in the presence of maltose, Casamino Acids, and Tween 80. In contrast to oxygen-labile activity, oxygen-stable activity was not detected in broth cultures.
鉴定了变形链球菌的β-溶血菌株，并研究了其生理反应和溶血素产生情况。结果表明，严格的厌氧条件对于在血琼脂平板上表达β-溶血是必不可少的；仅在氧气含量为10 ppm或更低的气氛中生长后才观察到溶血现象。发现β-溶血菌株的出现并不局限于变形链球菌的一种生物型。在液体培养物中检测到两种溶血活性。在预还原肉汤培养物中检测到对氧不稳定的活性，并且在培养上清液中该活性不稳定且无法检测到。通过向培养物中添加吐温80实现了对氧不稳定溶血素的溶解。在存在麦芽糖、酪蛋白氨基酸和吐温80的情况下，在细胞悬液的上清液中检测到对氧稳定的活性。与对氧不稳定的活性相反，在肉汤培养物中未检测到对氧稳定的活性。
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