胚胎小鼠小脑细胞在碳水化合物衍生化聚赖氨酸培养基质上的细胞组装模式。
Cell assembly patterns of embryonic mouse cerebellar cells on carbohydrate-derivatized polylysine culture substrata.
作者信息
Hatten M E
出版信息
J Cell Biol. 1981 Apr;89(1):54-61. doi: 10.1083/jcb.89.1.54.
Four carbohydrate derivatives of poly-D-lysine have been synthesized and assayed as substrates for the tissue culture of embryonic mouse cerebellar cells. On poly-beta-(D-glucopyranosyl)-poly-D-lysine and on poly-beta-(N-acetyl-D-glucosaminyl)-poly-D-lysine, dissociated cerebellar cells formed a monolayer. On poly-beta-(D-galactopyranosyl)-poly-D-lysine, cellular aggregates were formed and cables of processes were extended between the aggregates. On poly-beta-(L-fucosyl)-poly-D-lysine, cerebellar cells failed to attach and died within 24 h. On poly-(N-acetyl)-poly-D-lysine, cell attachment was identical to that on poly-D-lysine. At low concentrations of underivatized poly-D-lysine (0.5-2.0 microgram/ml) dissociated embryonic cerebellar cells formed cellular aggregates, whereas at higher concentrations of poly-D-lysine monolayering was extensive.
已合成了四种聚-D-赖氨酸的碳水化合物衍生物，并将其作为胚胎小鼠小脑细胞组织培养的底物进行了测定。在聚-β-(D-吡喃葡萄糖基)-聚-D-赖氨酸和聚-β-(N-乙酰-D-葡糖胺基)-聚-D-赖氨酸上，解离的小脑细胞形成了单层。在聚-β-(D-吡喃半乳糖基)-聚-D-赖氨酸上，形成了细胞聚集体，并且在聚集体之间延伸出突起束。在聚-β-(L-岩藻糖基)-聚-D-赖氨酸上，小脑细胞未能附着并在24小时内死亡。在聚-(N-乙酰)-聚-D-赖氨酸上，细胞附着情况与在聚-D-赖氨酸上相同。在低浓度的未衍生化聚-D-赖氨酸（0.5 - 2.0微克/毫升）下，解离的胚胎小脑细胞形成细胞聚集体，而在较高浓度的聚-D-赖氨酸下，单层形成广泛。
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