Katchaki J N, Siem T H, Brouwer R, Brandt K H, van der Waart M
J Virol Methods. 1980 Dec;2(1-2):119-25. doi: 10.1016/0166-0934(80)90045-2.
It has been suggested that post-transfusion hepatitis B (PTHB) may occur after transfusion with blood negative for hepatitis B surface antigen (HBsAg) but positive for antibody against hepatitis B core antigen (anti-HBc). We are currently conducting a controlled prospective study of recipients of such blood to investigate this possibility. Blood donors were routinely screened for HBsAg by radioimmunoassay (RIA) and those found negative were tested for anti-HBc by RIA. The HBsAg negative, anti-HBc positive donors were then tested by antibody directed against HBsAg by RIA, antibody directed against hepatitis B 'e' antigen by enzyme-immunoassay and for the liver enzymes SGOT and SGPT. To date,, follow-up has been completed in the recipients of 141 anti-HBc positive blood donors and in a control group of the recipients of 141 anti-HBc negative blood donors. All the recipients were bled repeatedly with intervals of 4--5 weeks up to 7 months post-transfusion. Currently, no clear-cut seroconversion was observed in recipients of either group for any of the antibody markers of HBV infection. While none of the 141 recipients of anti-HBc negative blood became positive for HBsAg, 3/141 recipients of anti-HBc positive blood acquired HBsAg and developed clinically manifest post-transfusion hepatitis B.
有人提出，在输注乙型肝炎表面抗原（HBsAg）阴性但乙型肝炎核心抗原抗体（抗-HBc）阳性的血液后，可能会发生输血后乙型肝炎（PTHB）。我们目前正在对接受此类血液的受血者进行一项对照前瞻性研究，以调查这种可能性。通过放射免疫测定法（RIA）对献血者进行常规HBsAg筛查，对检测结果为阴性的献血者再用RIA检测抗-HBc。然后，对HBsAg阴性、抗-HBc阳性的献血者，通过RIA检测其抗-HBs、通过酶免疫测定法检测其抗-HBe，并检测其肝酶谷草转氨酶（SGOT）和谷丙转氨酶（SGPT）。迄今为止，已完成了对141名抗-HBc阳性献血者的受血者以及141名抗-HBc阴性献血者的受血者组成的对照组的随访。所有受血者在输血后每隔4至5周反复采血，直至7个月。目前，在两组受血者中均未观察到任何HBV感染抗体标志物出现明确的血清学转换。141名抗-HBc阴性血液的受血者中无一例HBsAg转为阳性，而141名抗-HBc阳性血液的受血者中有3例获得了HBsAg，并发生了临床明显的输血后乙型肝炎。
