Fichman K R, Nyberg L M, Bujnovszky P, Brown T R, Walsh P C
J Clin Endocrinol Metab. 1981 May;52(5):919-23. doi: 10.1210/jcem-52-5-919.
To investigate the relationship between the androgen receptor content of human foreskin and age-dependent physiological changes in genital development, cytosolic and nuclear androgen receptors were measured in preputial skin specimens from male subjects of various ages. Optimum incubation conditions (4 C, 20 h) were established for measurement of androgen receptors by the exchange method with the synthetic androgen ligand methyltrienolone. The number of total androgen receptor sites, cytosolic plus nuclear, was fairly constant at all ages studied. However, the androgen receptor was predominantly localized to the nuclear compartment at those developmental stages characterized by higher levels of circulating blood androgens, i.e. newborns, pubertal males, and adults. By contrast, the androgen receptor in specimens from prepubertal boys was confined almost exclusively to the cytosolic compartment. This corresponds to a time of low plasma androgen levels and quiescence in genital maturation. It is suggested that changes in the intracellular distribution of androgen receptors may bear some relationship to the sequence of normal genital growth and development with nuclear androgen receptors having major physiological importance.
为了研究人类包皮雄激素受体含量与生殖器发育中年龄依赖性生理变化之间的关系，对不同年龄男性受试者的包皮皮肤标本中的胞质和核雄激素受体进行了测量。通过与合成雄激素配体甲基三烯olone的交换法测量雄激素受体，确定了最佳孵育条件（4℃，20小时）。在所研究的所有年龄组中，胞质加核的总雄激素受体位点数量相当恒定。然而，在以循环血液雄激素水平较高为特征的发育阶段，即新生儿、青春期男性和成年人中，雄激素受体主要定位于核区室。相比之下，青春期前男孩标本中的雄激素受体几乎完全局限于胞质区室。这与血浆雄激素水平低和生殖器成熟静止的时期相对应。提示雄激素受体内细胞分布的变化可能与正常生殖器生长发育的顺序有关，核雄激素受体具有主要的生理重要性。
