Festoff B W, Fernandez H L
Muscle Nerve. 1981 Jan-Feb;4(1):41-7. doi: 10.1002/mus.880040108.
Red blood cell and plasma cholinesterases were evaluated in control subjects and patients with the major forms of adult, sporadic motor neuron disease. For the purposes of this communication, the patients were considered as having amyotrophic lateral sclerosis (ALS) or its subtypes. Cholinesterase and acetylcholinesterase activities were evaluated and separated by dose response to their respective inhibitors. No kinetic differences were observed comparing red blood cell or plasma enzyme activities using either inhibitor. As found in previous studies, acetylcholinesterase accounted for more than 90% of acetylcholine hydrolysis in red blood cells. The plasma data were more complicated to evaluate, but at least 20% of total activity could be attributed to acetylcholinesterase. When red blood cell acetylcholinesterase activities of patients and controls were compared, no statistically significant difference was found. However, when plasma acetylcholinesterase activity was compared between the 2 groups, a statistically significant increase, almost twice the control value, was found in the ALS patients. These data may ultimately be important in the prognosis of this disease and, conceivably, could aid in understanding its pathogenesis.
对健康对照者以及患有主要类型的成人散发性运动神经元病患者的红细胞和血浆胆碱酯酶进行了评估。在本交流中，患者被视为患有肌萎缩侧索硬化症（ALS）或其亚型。对胆碱酯酶和乙酰胆碱酯酶活性进行了评估，并通过对各自抑制剂的剂量反应进行分离。使用任何一种抑制剂比较红细胞或血浆酶活性时，均未观察到动力学差异。如先前研究中所发现的，乙酰胆碱酯酶在红细胞中占乙酰胆碱水解的90%以上。血浆数据评估起来更为复杂，但总活性中至少20%可归因于乙酰胆碱酯酶。比较患者和对照者的红细胞乙酰胆碱酯酶活性时，未发现统计学上的显著差异。然而，比较两组之间的血浆乙酰胆碱酯酶活性时，发现ALS患者有统计学上的显著升高，几乎是对照值的两倍。这些数据最终可能对该疾病的预后很重要，并且可以想象，有助于理解其发病机制。
