用胰蛋白酶消化法分析血影蛋白的主要多肽。

Analysis of the major polypeptides of spectrin by tryptic digestion.

作者信息

Maddy A H, Dunn M J

出版信息

J Supramol Struct. 1978;8(4):465-71. doi: 10.1002/jss.400080409.

Abstract

The two major polypeptides of erythrocyte membrane spectrin have been isolated by preparative polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. The tryptic peptide maps of the two polypeptides have been prepared by thin-layer chromatography and electrophoresis. Radioactive peptides have been prepared by 14C-carboxymethylation and chloramine T-catalysed 125I iodination. Maps of both sets of peptides demonstrate a marked similarity between the two parent polypeptides.

摘要

红细胞膜血影蛋白的两种主要多肽已通过在十二烷基硫酸钠存在下的制备性聚丙烯酰胺凝胶电泳分离出来。这两种多肽的胰蛋白酶肽图已通过薄层色谱法和电泳法制备。放射性肽已通过14C-羧甲基化和氯胺T催化的125I碘化制备。两组肽的图谱都显示出两种亲本多肽之间有明显的相似性。