在磷脂悬浮液存在的情况下，用胰蛋白酶和链霉蛋白酶对血影蛋白进行蛋白水解。
Proteolysis of spectrin by trypsin and pronase in the presence of phospholipid suspensions.
作者信息
Sikorski A F, Kozubek A, Szopa J
机构信息
Institute of Biochemistry, Wrocław University, Poland.
出版信息
Acta Biochim Pol. 1988;35(2):71-81.
The effect of phospholipid suspensions on the proteolysis of isolated spectrin was examined by SDS-polyacrylamide gradient gel electrophoresis. Proteolysis of spectrin in the membranes by trypsin and pronase was also studied. It was found that electrophoretic patterns of spectrin fragments were influenced by the presence of the suspension prepared from phosphatidylethanolamine:phosphatidylserine (60:40) mixture and of phosphatidylcholine. Qualitative changes in the proteolytic patterns obtained after proteolysis of spectrin by pronase in the presence of phosphatidylcholine suspension were observed. The changes in the sensitivity of spectrin towards proteases result probably from changes in the accessibility of some peptide bonds upon the interaction of this extrinsic protein with phospholipids.
通过SDS-聚丙烯酰胺梯度凝胶电泳检测了磷脂悬浮液对分离的血影蛋白蛋白水解作用的影响。还研究了胰蛋白酶和链霉蛋白酶对膜中血影蛋白的蛋白水解作用。发现由磷脂酰乙醇胺：磷脂酰丝氨酸（60:40）混合物和磷脂酰胆碱制备的悬浮液的存在会影响血影蛋白片段的电泳图谱。观察到在磷脂酰胆碱悬浮液存在下，链霉蛋白酶对血影蛋白进行蛋白水解后，蛋白水解模式发生了定性变化。血影蛋白对蛋白酶敏感性的变化可能是由于这种外在蛋白与磷脂相互作用时某些肽键的可及性发生了变化。
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