Fidler I J, Raz A, Fogler W E, Poste G
Recent Results Cancer Res. 1980;75:246-51. doi: 10.1007/978-3-642-81491-4_38.
Clearance from the circulation and tissue distribution of liposomes of differing size, surface charge, and composition injected IV into BALB/c mice have been examined. Large (500-3,400 nm diameter) liposomes are cleared from the circulation more rapidly than small sonicated liposomes (less than 100 nm diameter). Negatively charged liposomes are cleared more rapidly than neutral or positively charged liposomes of the same size. The major site at which liposomes injected IV accumulate, irrespective of their composition, is the liver. Accumulation of liposomes at extrahepatic sites, such as the lung, is influenced by liposome size, charge, and composition. Optimal localization and retention of liposomes in the lung were achieved with large negatively charged multilamellar and reversed evaporation phase liposomes containing phosphatidylserine (PS/PC, 3:7 mol ratio; PS/PC/LL 4.95:4.95:0.1 mol ratio).
已对静脉注射到BALB/c小鼠体内的不同大小、表面电荷和组成的脂质体的循环清除率和组织分布进行了研究。大的（直径500 - 3400 nm）脂质体比小的超声处理脂质体（直径小于100 nm）从循环中清除得更快。带负电荷的脂质体比相同大小的中性或带正电荷的脂质体清除得更快。静脉注射的脂质体无论其组成如何，主要积聚部位是肝脏。脂质体在肝外部位（如肺）的积聚受脂质体大小、电荷和组成的影响。含有磷脂酰丝氨酸（PS/PC，摩尔比3:7；PS/PC/LL，摩尔比4.95:4.95:0.1）的大的带负电荷的多层和逆相蒸发相脂质体在肺中实现了最佳定位和滞留。
