Blomquist E, Fredriksson B A, Brunk U
Ultrastruct Pathol. 1980 Jan-Mar;1(1):11-7. doi: 10.3109/01913128009141391.
Secondary lysosomes of the residual body type are frequent in nondividing cells from phase III cultures of human glial cells. These organelles have previously been shown to be analogous to lipofuscin granules of postmitotic cells in vivo. Most recent studies favor the assumption that residual bodies mainly result from incomplete degradation within the lysosomal vacuome of endogenous cellular components such as mitochondria and endoplasmic reticulum. Since iron occurs in several metalloenzymes produced by such organelles, it should then be possible to demonstrate accumulated iron within residual bodies. X-ray dispersive analysis of sectioned biological material is often hampered by diffusion and dissolution during preparation, as well as by too low a concentration of the elements. In this study we cultured glial cells on Formvar-coated gold grids and studied them unsectioned, after brief glutaraldehyde fixation and freeze-drying, in a transmission electron microscope at 100 kV in TEM and STEM mode. It was then possible to demonstrate iron in residual bodies of aged cells, presumably because the type of preparation utilized does not permit much dissolution.
在人神经胶质细胞III期培养的非分裂细胞中，残留体类型的次级溶酶体很常见。这些细胞器先前已被证明类似于体内有丝分裂后细胞的脂褐素颗粒。最近的大多数研究倾向于这样一种假设，即残留体主要是由于内源性细胞成分（如线粒体和内质网）在溶酶体液泡系统内不完全降解所致。由于铁存在于这些细胞器产生的几种金属酶中，那么应该有可能在残留体内证明铁的积累。对切片生物材料进行X射线色散分析时，常常会因制备过程中的扩散和溶解以及元素浓度过低而受到阻碍。在本研究中，我们将神经胶质细胞培养在涂有福尔马弗的金网上，在经过短暂戊二醛固定和冷冻干燥后，不进行切片，直接在100 kV的透射电子显微镜下以TEM和STEM模式进行研究。这样就有可能在衰老细胞的残留体内证明铁的存在，大概是因为所采用的制备方法不会导致太多的溶解。
