Ruigrok T J, de Moes D, Slade A M, Nayler W G
Am J Pathol. 1981 Jun;103(3):390-403.
Reperfusion of isolated rat hearts with calcium-containing solution after a short period of calcium-free perfusion results in irreversible cell damage (calcium paradox). Experiments were undertaken to study the effect of dimethylsulfoxide (DMSO) on the occurrence of the calcium paradox in rat heart muscle. DMSO (1.4 mol/l) was added to the calcium-free or the reperfusion medium. Cell damage was quantitated in terms of creatine kinase (CK) release, cardiac electrogram (CEG) changes, and ultrastructural damage. Reperfusion with calcium-containing solution without DMSO after calcium-free perfusion with DMSO resulted in massive release of CK, electrical arrest of the ventricles, and formation of contraction bands. Nearly 100% of the cells displayed the calcium paradox damage. Reperfusion with calcium-containing solution with DMSO after calcium-free perfusion without DMSO resulted in a moderate release of CK, and electrical arrest of the ventricles. The myofibrils remained in a relaxed state. Only 4% of the cells displayed the calcium paradox damage. These results indicate that DMSO does not protect rat hearts against the effects of calcium-free perfusion that predispose the myocardium to the calcium paradox. The calcium paradox damage is reduced, however, when DMSO is present during the reperfusion phase.
在短时间无钙灌注后用含钙溶液对离体大鼠心脏进行再灌注会导致不可逆的细胞损伤（钙反常）。进行实验以研究二甲基亚砜（DMSO）对大鼠心肌钙反常发生的影响。将DMSO（1.4 mol/l）添加到无钙或再灌注培养基中。通过肌酸激酶（CK）释放、心电图（CEG）变化和超微结构损伤来定量细胞损伤。在用DMSO进行无钙灌注后，用不含DMSO的含钙溶液进行再灌注会导致CK大量释放、心室电停搏和收缩带形成。近100%的细胞表现出钙反常损伤。在用不含DMSO的溶液进行无钙灌注后，用含DMSO的含钙溶液进行再灌注会导致CK适度释放和心室电停搏。肌原纤维保持松弛状态。只有4%的细胞表现出钙反常损伤。这些结果表明，DMSO不能保护大鼠心脏免受无钙灌注的影响，而无钙灌注会使心肌易发生钙反常。然而，当在再灌注阶段存在DMSO时，钙反常损伤会减轻。
