一项关于组织培养中人类肿瘤细胞生长需求的研究。

A study of the growth requirements of human tumour cells in tissue culture.

作者信息

Rowbottom L A, Whitehead R H, Roberts G P, Hughes L E

出版信息

Aust J Exp Biol Med Sci. 1981 Feb;59(1):91-100. doi: 10.1038/icb.1981.5.

DOI:10.1038/icb.1981.5

Abstract

The effect of potential growth enhancing substances and other environmental factors on the growth of human cells in culture were examined. For all the cell lines tested the optimum pH range was 7 . 45-7 . 55. Optimum osmolality varied with different cell lines, optimum values being 240 milliosmoles/kg for melanoma cell lines and foetal fibroblasts, 260 milliosmoles/kg for adult fibroblasts and 300 milliosmoles/kg for a breast cancer cell line. A number of hormones were found to affect the growth of the cell lines examined. Insulin and hydrocortisone enhanced the growth of all the tumour cells tested, whereas 17 beta-oestradiol enhanced the growth of breast cancer cells but inhibited the growth of melanoma cells and fibroblasts. Stilboestrol inhibited the growth of all the cell lines but prolactin enhanced the growth of melanoma cells and inhibited fibroblast cell growth. Addition of galactose to the medium stimulated the growth of all cell lines tested, whereas sucrose and fructose resulted in stimulation of tumour cell growth but had little effect on fibroblasts. alpha-thioglycerol and beta-mercaptoethanol enhanced the growth of the tumour cells but had little effect on the growth of fibroblasts. A modified medium has been formulated which enhanced the growth of all the cell lines used when compared with McCoy's 5A medium.

摘要

研究了潜在的生长促进物质和其他环境因素对培养的人类细胞生长的影响。对于所有测试的细胞系，最佳pH范围是7.45 - 7.55。最佳渗透压因不同细胞系而异，黑色素瘤细胞系和胎儿成纤维细胞的最佳值为240毫渗摩尔/千克，成人成纤维细胞为260毫渗摩尔/千克，乳腺癌细胞系为300毫渗摩尔/千克。发现多种激素会影响所检测细胞系的生长。胰岛素和氢化可的松促进了所有测试肿瘤细胞的生长，而17β - 雌二醇促进了乳腺癌细胞的生长，但抑制了黑色素瘤细胞和成纤维细胞的生长。己烯雌酚抑制了所有细胞系的生长，但催乳素促进了黑色素瘤细胞的生长并抑制了成纤维细胞的生长。向培养基中添加半乳糖刺激了所有测试细胞系的生长，而蔗糖和果糖则促进了肿瘤细胞的生长，但对成纤维细胞影响不大。α - 硫甘油和β - 巯基乙醇促进了肿瘤细胞的生长，但对成纤维细胞的生长影响不大。已配制出一种改良培养基，与 McCoy's 5A 培养基相比，该培养基促进了所有所用细胞系的生长。