Lindblom G, Johansson L B, Arvidson G
Biochemistry. 1981 Apr 14;20(8):2204-7. doi: 10.1021/bi00511a020.
Lateral diffusion coefficients of lipids in a bilayer can be measured directly in a macroscopically aligned sample by use of a pulsed NMR method with pulsed magnetic field gradients [Lindblom, G., & Wennerström, H. (1977) Biophys. Chem. 6, 167]. This technique has been utilized to investigate the influence of cholesterol on the lipid diffusion of egg yolk lecithin, palmitoyloleoyllecithin, and dioleoyllecithin. It is found that cholesterol has a very small effect on the phospholipid diffusion. On the other hand, cholesterol has a great influence on the molecular ordering in the bilayer and on the lipid phase structure. It is therefore suggested that cholesterol exerts its dominant effect on the lipid membrane stability.
通过使用带有脉冲磁场梯度的脉冲核磁共振方法，可以在宏观取向的样品中直接测量双层中脂质的横向扩散系数[林德布洛姆，G.，& 温纳斯特伦，H.（1977年）《生物物理化学》6，167]。该技术已被用于研究胆固醇对蛋黄卵磷脂、棕榈酰油酰卵磷脂和二油酰卵磷脂脂质扩散的影响。发现胆固醇对磷脂扩散的影响非常小。另一方面，胆固醇对双层中的分子有序性和脂质相结构有很大影响。因此，有人认为胆固醇对脂质膜稳定性起主要作用。
