Svec F, Rudis M
Biochim Biophys Acta. 1981 Apr 17;674(1):30-6. doi: 10.1016/0304-4165(81)90343-3.
Although glucocorticoids influence pancreatic function, it has not been established whether they act directly at the level of the pancreas, or indirectly by causing metabolic changes in other target tissues. As a step in elucidating the actions of glucocorticoids on the pancreas, a search was conducted for glucocorticoid hormone receptors in this tissue. Uptake and binding studies indicated that there were glucocorticoid hormone receptors in the high-speed cytosolic extract of rat pancreas. These receptors appear to be similar to other rat glucocorticoid receptors: they bind glucocorticoids rapidly in a reversible manner at 0 degrees C, competitive binding analysis studies show that they have a preference for glucocorticoids and, like other receptors, bind the synthetic steroids triamcinolone acetonide and dexamethasone with a higher affinity than corticosterone. Scatchard analysis demonstrated that there are 1.37 . 10(-13) mol glucocorticoid-binding sites/mg cytosolic protein. This demonstration of a glucocorticoid hormone receptor in pancreatic cytosol suggests that some of the effects glucocorticoids exert on pancreatic function are a consequence of their direct actions on this target tissue.
尽管糖皮质激素会影响胰腺功能，但它们是直接作用于胰腺，还是通过引起其他靶组织的代谢变化而间接发挥作用，目前尚无定论。作为阐明糖皮质激素对胰腺作用的一个步骤，我们对该组织中的糖皮质激素受体进行了研究。摄取和结合研究表明，大鼠胰腺的高速胞质提取物中存在糖皮质激素受体。这些受体似乎与其他大鼠糖皮质激素受体相似：它们在0℃时以可逆方式快速结合糖皮质激素，竞争性结合分析研究表明它们对糖皮质激素有偏好，并且与其他受体一样，与合成类固醇曲安奈德和地塞米松的结合亲和力高于皮质酮。Scatchard分析表明，每毫克胞质蛋白中有1.37×10⁻¹³摩尔糖皮质激素结合位点。胰腺胞质溶胶中糖皮质激素受体的这一证明表明，糖皮质激素对胰腺功能的某些影响是它们直接作用于该靶组织的结果。
