Mori K J, Izumi H, Seto A
Biomedicine. 1981 Mar;35(1):16-8.
Long-term hemopoiesis was established in bone marrow cell culture in vitro. This culture was shown to support the recovery proliferation of hemopoietic stem cells completely in vitro after irradiation. Hemopoietic stem cells were stimulated into proliferation in culture when normal bone marrow cells were overlayed on top of the irradiated adherent cell colonies. These results indicate that proliferation and differentiation of hemopoietic stem cells in vitro are also supported by stroma-hemopoietic cell interactions.
长期造血在体外骨髓细胞培养中得以建立。该培养体系被证明能够在体外完全支持受照射后造血干细胞的恢复性增殖。当将正常骨髓细胞覆盖在受照射的贴壁细胞集落上时，造血干细胞在培养中被刺激增殖。这些结果表明，基质-造血细胞相互作用也支持造血干细胞在体外的增殖和分化。
