O'Donnell R W, Cockerell G L
Cancer Res. 1981 Jun;41(6):2372-7.
A colonic adenocarcinoma cell line, designated GPC-16, was established in culture from tissue removed from an outbred guinea pig which received 56 mg of N-methyl-N-nitrosourea intrarectally over a 28-week period. the GPC-16 has been maintained for more than 30 in vitro passages and was tumorigenic in both the autochthonous host and nude mice. The cells were aneuploid with a wide range in chromosome number (54 to 172) in a bimodal distribution. The cells grew in vitro as a tight monolayer and demonstrated numerous desmosomes by electron microscopy. In vitro-passaged cells had a doubling time of 36 hr and a 5.1% plating efficiency in agar.
一种名为GPC - 16的结肠腺癌细胞系，是从一只远交豚鼠切除的组织中培养建立的，该豚鼠在28周内直肠内接受了56毫克N - 甲基 - N - 亚硝基脲。GPC - 16已在体外传代30次以上，在同种宿主和裸鼠中均具有致瘤性。细胞为非整倍体，染色体数目范围广泛（54至172条），呈双峰分布。细胞在体外生长形成紧密的单层，通过电子显微镜观察可见大量桥粒。体外传代的细胞倍增时间为36小时，在琼脂中的接种效率为5.1%。
