Artiss J D, Thibert R J, Zak B
Clin Biochem. 1981 Feb;14(1):32-8. doi: 10.1016/0009-9120(81)90165-x.
A spectrophotometric study was carried out for three proteins assays when modification of their serum procedures using bromcresol green, bromcresol purple and biuret reagents were applied to the determinations of total proteins and albumin in cerebrospinal fluids. A novel concentration device wherein the sample itself was used as the primary diluent for the three reagents concentrated to contain the proper amounts of chemicals in smaller volumes than suggested in their serum procedures allowed reasonable absorbance signals to be obtained. Low molecular weight molecules were separated from the albumin and globulins of the fluids by centrifugal ultrafiltration using a 25K cutoff and spectra were obtained for both high and low molecular weight fractions. Some materials were obtained in the separated ultrafiltrates which gave reactions with all three reagents, reactions which either overlapped the spectra of the albumin reactions or superimposed the spectra obtained with the total protein reaction. A screening procedure for cerebrospinal fluid total proteins or centrifugally ultrafiltered albumin appears reasonable as an inference from studies made, although further elucidation of the low molecular weight fractions in needed as a confirmation device.
当使用溴甲酚绿、溴甲酚紫和双缩脲试剂对血清检测程序进行改进以测定脑脊液中的总蛋白和白蛋白时，开展了一项针对三种蛋白质检测方法的分光光度研究。一种新型浓缩装置，其中样本本身用作三种试剂的主要稀释剂，浓缩后的试剂含有适量化学物质，体积比其血清检测程序中建议的更小，从而能够获得合理的吸光度信号。使用截留分子量为25K的离心超滤法将低分子量分子与脑脊液中的白蛋白和球蛋白分离，并获得了高分子量和低分子量组分的光谱。在分离出的超滤物中得到了一些物质，它们与所有三种试剂都发生反应，这些反应要么与白蛋白反应的光谱重叠，要么叠加了总蛋白反应得到的光谱。根据已开展的研究推断，脑脊液总蛋白或离心超滤白蛋白的筛查程序似乎是合理的，不过作为一种确认手段，还需要进一步阐明低分子量组分。
