Bannister S J, van der Wal S, Dolan J W, Snyder L R
Clin Chem. 1981 Jun;27(6):849-55.
We describe a single procedure for assay of seven tricyclic antidepressant drugs and metabolites in serum or plasma: protriptyline, nortriptyline, amitriptyline, desmethyldoxepin, doxepin, desipramine, and imipramine. With the Technicon "FAST-LC" system, samples are aspirated directly into the unit and pretreated via double extraction; the concentration of each drug is then determined by "high-performance" liquid chromatography. Final chromatograms are monitored at 205 nm, at analysis rates of 7.5 samples/h. Concentration and absorbance are linearly related for each drug from 0 to 1400 micrograms/L. Day-to-day CVs averaged 5 to 6% for each drug, and there is good correlation of FAST-LC values with those obtained by gas-chromatographic methods. Total sample volume is 750 microliters.
普罗替林、去甲替林、阿米替林、去甲基多塞平、多塞平、地昔帕明和丙咪嗪。使用Technicon“FAST-LC”系统，样品直接吸入该装置并通过双重萃取进行预处理；然后通过“高效”液相色谱法测定每种药物的浓度。最终色谱图在205nm处监测，分析速率为每小时7.5个样品。每种药物在0至1400微克/升范围内，浓度与吸光度呈线性关系。每种药物的日间变异系数平均为5%至6%，且FAST-LC值与气相色谱法获得的值具有良好的相关性。总样品体积为750微升。
