Durnam D M, Palmiter R D
J Biol Chem. 1981 Jun 10;256(11):5712-6.
Administration of Cd, Zn, Cu, or Hg increases the rate of transcription from the metallothionein-I gene in mouse liver and kidney. Maximal transcription rates occur 1 h after Cd administration in both tissues. Metallothionein-I mRNA levels, measured by cDNA hybridization, and metallothionein protein synthesis, measured by [35S]cysteine incorporation, increase simultaneously, reaching maximal levels about 4 h after Cd administration. Cd also induces metallothionein-I mRNA in all other tissues examined (spleen, heart, skeletal muscle, brain, and intestine) except testes. Comparison of the inductions by Cd and Hg shows that the kinetics of metallothionein-I mRNA accumulation as well as the stability of the resultant metallothioneins differ.
给予镉、锌、铜或汞会提高小鼠肝脏和肾脏中金属硫蛋白-I基因的转录速率。在这两种组织中，镉给药1小时后会出现最大转录速率。通过cDNA杂交测量的金属硫蛋白-I mRNA水平以及通过[35S]半胱氨酸掺入测量的金属硫蛋白蛋白质合成同时增加，在镉给药后约4小时达到最大水平。镉还能在除睾丸外的所有其他检测组织（脾脏、心脏、骨骼肌、大脑和肠道）中诱导金属硫蛋白-I mRNA。镉和汞诱导作用的比较表明，金属硫蛋白-I mRNA积累的动力学以及所产生的金属硫蛋白的稳定性有所不同。
