Durnam D M, Hoffman J S, Quaife C J, Benditt E P, Chen H Y, Brinster R L, Palmiter R D
Proc Natl Acad Sci U S A. 1984 Feb;81(4):1053-6. doi: 10.1073/pnas.81.4.1053.
++Induction of metallothionein-I (MT-I) mRNA by bacterial endotoxin (LPS) was examined. A single injection of LPS induced MT-I mRNA accumulation in both liver and kidney comparable to that induced by heavy metals. Maximal message levels were achieved 6 hr after LPS administration, prior to any increase in either hepatic or renal Zn or Cu. Experiments in which LPS was administered to transgenic mice harboring recombinant genes made by fusing the MT-I gene promoter to the herpes simplex virus thymidine kinase structural gene revealed that the response to LPS is independent of glucocorticoid hormones. These experiments begin to define the region of the MT-I gene promoter required for the LPS response.
研究了细菌内毒素（LPS）对金属硫蛋白-I（MT-I）mRNA的诱导作用。单次注射LPS可诱导肝脏和肾脏中MT-I mRNA积累，其水平与重金属诱导的相当。LPS给药后6小时达到最大信息水平，此时肝脏或肾脏中的锌或铜均未增加。将MT-I基因启动子与单纯疱疹病毒胸苷激酶结构基因融合构建重组基因，并将LPS注射到携带该重组基因的转基因小鼠体内进行实验，结果显示对LPS的反应不依赖于糖皮质激素。这些实验开始确定LPS反应所需的MT-I基因启动子区域。
