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Human immunoglobulin M purification by affinity chromatography on protein A--Sepharose.

作者信息

Vidal M A, Conde F P

出版信息

J Biochem Biophys Methods. 1981 Mar;4(3-4):155-61. doi: 10.1016/0165-022x(81)90052-x.

Abstract

A method is described for preparing IgM from normal serum in two chromatographic steps. A gel filtration column gives a macroglobulin peak containing the IgM, purified by affinity chromatography on protein A--Sepharose. Immunoglobulin recovery is around 50%.

摘要

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