Vidal M A, Conde F P
J Biochem Biophys Methods. 1981 Mar;4(3-4):155-61. doi: 10.1016/0165-022x(81)90052-x.
A method is described for preparing IgM from normal serum in two chromatographic steps. A gel filtration column gives a macroglobulin peak containing the IgM, purified by affinity chromatography on protein A--Sepharose. Immunoglobulin recovery is around 50%.