通过冻融法在蔗糖梯度上进行多核糖体分析。

Polyribosome analysis on sucrose gradients produced by the freeze-thaw method.

作者信息

Fourcroy P, Cuiller S, Largitte F C, Lambert C

出版信息

J Biochem Biophys Methods. 1981 Mar;4(3-4):243-6. doi: 10.1016/0165-022x(81)90063-4.

Abstract

Polyribosomes were analyzed on sucrose gradients produced by the freeze-thaw method. When gradients were formed from a starting solution containing 1 mM Mg2+ an anomalous sedimentation pattern was observed although the polyribosomal structure can be maintained at this Mg2+ concentration. The use of starting solutions containing 5--20 mM Mg2+ yielded a satisfactory sedimentation profile of polyribosomes on freeze-thaw produced gradients.

摘要

采用冻融法制备蔗糖梯度对多核糖体进行分析。当从含有1 mM Mg2+的起始溶液形成梯度时，尽管在该Mg2+浓度下多核糖体结构能够维持，但观察到了异常的沉降模式。使用含有5 - 20 mM Mg2+的起始溶液，在冻融法制备的梯度上得到了令人满意的多核糖体沉降图谱。