豚鼠耳表皮中鸟氨酸脱羧酶活性随创伤变化的动力学。

The kinetics of ornithine decarboxylase activity as a function of wounding in guinea pig ear epidermis.

作者信息

Bolton L L, Constantine B E, Rovee D T

出版信息

J Invest Dermatol. 1981 Jun;76(6):480-3. doi: 10.1111/1523-1747.ep12521171.

Abstract

Guinea pig ear stratum corneum was removed to increasing depths by 1, 3, 6 or 9 strippings with adhesive tape. Increasing ornithine decarboxylase (ODC) activity responses were observed in the epidermis 4.5 hr after wounding with 3, 6 or 9 tape strippings. The kinetics of this ODC response were investigated at intervals including, 2, 4.5, 12, 18, 24 and 72 hr after tape stripping. ODC activity was significantly elevated for 12 hr after 3 or 6 tape strippings and for at least 72 hr after 9 tape strippings. These effects were independent of hair plucking or depilation prior to wounding. This model is potentially useful for comparing the effects of chemical, mechanical and physical stimuli on ODC activity and subsequent polyamine synthesis.

摘要

用胶带对豚鼠耳部角质层进行1、3、6或9次剥离，以逐渐增加去除深度。在用胶带进行3、6或9次剥离造成创伤后4.5小时，观察到表皮中鸟氨酸脱羧酶（ODC）活性反应增强。在胶带剥离后的2、4.5、12、18、24和72小时等时间间隔对这种ODC反应的动力学进行了研究。在进行3次或6次胶带剥离后，ODC活性显著升高12小时，在进行9次胶带剥离后至少72小时保持升高。这些效应与创伤前拔毛或脱毛无关。该模型对于比较化学、机械和物理刺激对ODC活性及随后多胺合成的影响可能具有潜在用途。