Fujita Y, Mori T, Aso T, Nishimura T
Nihon Sanka Fujinka Gakkai Zasshi. 1980 Jun;32(6):706-12.
T elucidate the possible intraovarian site for androgen overproduction in the polycystic ovary (PCO), we investigated relative contribution of follicular and stromal compartments in steroidogenesis by in vitro experiments. The largest follicle and stoma were dissected from the PCO tissue obtained by wedge resection. A portion of each tissue was taken for histology and slices of each tissue were incubated with acetate-1-14C for 3 hours. Incorporation into progestins, androgens and estrogens was assessed by the reverse isotope dilution technique. Qualitatively, a predominant incorporation into androstenedione (76-88%) with little incorporation into estrogens (0.6-2.5%) was commonly observed in the follicle with marked hyperthecosis found in 3 typical PCOs, whereas a lesser incorporation into androstenedione (51-57%) with a slight increase into estrogens (6.4-14.6%) was verified in the follicles with mild hyperthecosis in 2 atypical PCOs. Quantitatively, actual incorporation into androstenedione was the greatest in the typical PCO follicles, followed by the atypical PCO follicles and the least or almost negligible in the stroma of PCOs. We concluded that atretic follicles rather than the stroma are principal sites of androgen overproduction by PCOs, and the androgenic capacity is related positively to the grade of hyperthecosis.
为了阐明多囊卵巢（PCO）中雄激素过度产生的可能的卵巢内位点，我们通过体外实验研究了卵泡和间质部分在类固醇生成中的相对作用。从楔形切除获得的PCO组织中解剖出最大的卵泡和间质。将每个组织的一部分用于组织学检查，并将每个组织的切片与乙酸盐-1-14C孵育3小时。通过反向同位素稀释技术评估其掺入孕激素、雄激素和雌激素的情况。定性地，在3个典型PCO中发现的有明显卵泡膜细胞增生的卵泡中，通常观察到主要掺入雄烯二酮（76-88%），而雌激素掺入很少（0.6-2.5%），而在2个非典型PCO中轻度卵泡膜细胞增生的卵泡中，雄烯二酮掺入较少（51-57%），雌激素略有增加（6.4-14.6%）。定量地，典型PCO卵泡中实际掺入雄烯二酮的量最大，其次是非典型PCO卵泡，而PCO间质中的量最少或几乎可以忽略不计。我们得出结论，闭锁卵泡而非间质是PCO雄激素过度产生的主要位点，并且雄激素生成能力与卵泡膜细胞增生的程度呈正相关。
