Nadakavukaren K K, Rempfer G F, Griffith O H
J Microsc. 1981 Jun;122(Pt 3):301-7. doi: 10.1111/j.1365-2818.1981.tb01270.x.
Photoelectron micrographs of gold-palladium coated mouse 3T3 cells and chick embryo fibroblasts are presented. Since the gold-palladium suppresses differences in work function, the cell morphology seen in these micrographs is due to relief contrast. The heights of comparable cells were measured from the parallax present in transmission electron micrograph stereo-pairs of cell surface replicas. The origin of relief contrast and the effect of cell surface relief on the working depth of field in photoelectron images of cells are discussed. The micrographs demonstrate that photoelectron microscopy is very sensitive to fine details of cell surface topography, and that the working depth of field is not a limiting factor in the imaging of well-spread tissue culture cells.
展示了金钯涂层小鼠3T3细胞和鸡胚成纤维细胞的光电子显微照片。由于金钯抑制了功函数的差异，这些显微照片中看到的细胞形态是由于浮雕对比度。从细胞表面复制品的透射电子显微镜立体对中的视差测量了可比细胞的高度。讨论了浮雕对比度的起源以及细胞表面浮雕对细胞光电子图像中工作景深的影响。显微照片表明，光电子显微镜对细胞表面形貌的细微细节非常敏感，并且工作景深不是铺展良好的组织培养细胞成像的限制因素。
