[来自短密青霉的细胞内核糖核酸酶及其天然抑制剂]
[Intracellular ribonucleases from Penicillin brevicompactum and their natural inhibitors].
作者信息
Zigel' M, Bezborodov A M
出版信息
Prikl Biokhim Mikrobiol. 1981 Mar-Apr;17(2):203-9.
PMID:7243745
Abstract
The ability of the fungus Penicillium brevi-compactum for the synthesis of intracellular ribonucleases and their inhibitors was examined. Activities of ribonucleases and their inhibitors were found to vary with the culture growth. As a result of isopropanol precipitation of the cell-free homogenate, ion-exchange chromatography on DEAE-cellulose and gel filtration on Sephadex G-75, two fractions were obtained, they being inhibitor complexes with acid and alkaline intracellular ribonucleases.
摘要
对短密青霉合成细胞内核糖核酸酶及其抑制剂的能力进行了研究。发现核糖核酸酶及其抑制剂的活性随培养物生长而变化。通过对无细胞匀浆进行异丙醇沉淀、在DEAE - 纤维素上进行离子交换色谱以及在Sephadex G - 75上进行凝胶过滤，得到了两个级分，它们是与酸性和碱性细胞内核糖核酸酶的抑制剂复合物。
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