分离细胞核中的RNA合成：汞化核苷酸的应用。
RNA synthesis in isolated nuclei: the use of mercurated nucleotides.
作者信息
Mory Y, Gefter M
出版信息
Nucleic Acids Res. 1978 Oct;5(10):3899-912. doi: 10.1093/nar/5.10.3899.
Abstract
We have used uridine 5' triphosphate-5-mercury (Hg-UTP) in place of UTP to study RNA synthesis in a previously described isolated nuclei system (1). Employing isopycnic density gradient centrifugation to separate RNAs based upon their relative content of Hg-U, several conclusions can be drawn. In vitro RNA synthesis consists of end addition onto pre-initiated HnRNA molecules as well as apparent initiation of new HnRNA molecules de novo. Synthesis in our system continues linearly for greater than two hours. The chain elongation rate has been measured to be about 500 nucleotides per minute. The methods used to make these measurements are generally applicable to other in vitro systems.
摘要
我们使用尿苷5′-三磷酸-5-汞（Hg-UTP）代替UTP，以研究先前描述的分离细胞核系统中的RNA合成（1）。利用等密度梯度离心根据RNA中Hg-U的相对含量来分离RNA，可以得出几个结论。体外RNA合成包括在预先起始的核不均一RNA（HnRNA）分子上进行末端添加，以及明显地从头起始新的HnRNA分子。我们系统中的合成持续线性进行超过两小时。已测得链延伸速率约为每分钟500个核苷酸。用于进行这些测量的方法通常适用于其他体外系统。
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