Crouse G F, Fodor J B, Doty P
Proc Natl Acad Sci U S A. 1976 May;73(5):1564-7. doi: 10.1073/pnas.73.5.1564.
Mercurated uridine triphosphate has been used to label transcripts of chicken reticulocyte chromatin made with Escherichia coli RNA polymerase. The mercury-labeled RNA product can be completely separated from endogenous RNA sequences in the chromatin by passage through a sulfhydryl Sepharose column. Globin cDNA hybridization to the transcript shows that only 2.6 x 10-5 of the transcript is globin RNA. In contrast to this result, erythrocte chromatin transcript contains less than one tenth as many globin RNA sequences.
汞化尿苷三磷酸已被用于标记用大肠杆菌RNA聚合酶合成的鸡网织红细胞染色质的转录本。通过巯基琼脂糖柱,可将汞标记的RNA产物与染色质中的内源性RNA序列完全分离。珠蛋白cDNA与转录本的杂交显示,转录本中只有2.6×10⁻⁵是珠蛋白RNA。与该结果相反,红细胞染色质转录本中珠蛋白RNA序列的数量不到其十分之一。