烟草花叶病毒蛋白与含荧光标记的合成多核苷酸的相互作用：聚（A，εA）和聚（C，εC）共聚物的光学性质以及核糖核蛋白中色氨酸到1，N6-乙烯基腺嘌呤或3，N4-乙烯基胞嘧啶残基的能量迁移
Interaction of tobacco mosaic virus protein with synthetic polynucleotides containing a fluorescent label: optical properties of poly(A,epsilonA) and poly(C,epsilonC) copolymers and energy migration from the tryptophan to 1,N6-ethenoadenine or 3,N4-ethenocytosine residues in RNP.
作者信息
Ledneva R K, Razjivin A P, Kost A A, Bogdanov A A
出版信息
Nucleic Acids Res. 1978 Nov;5(11):4225-43. doi: 10.1093/nar/5.11.4225.
Abstract
A spectrophotometric method for determination of the modification degrees and molar extinction coefficients for poly(A, epsilonA) and poly(C, epsilonC) copolymers has been developed. Dependence of some absorption and fluorescence parameters of the copolymers on the modification degree has been studied. Distribution of modified residues in copolymers differs from random and depends on modification conditions. Interaction between the TMV protein and copolymers has been investigated. The protein interacts with poly(A, epsilonA) of low or medium modification degree and displays no activity with respect to poly(epsilonA). On the contrary, introduction of epsilonC to the polynucleotide promotes complex formation between poly(C, epsilonC) and TMV protein. Analysis of the fluorescence emission and excitation spectra has revealed energy transfer from tryptophan to epsilonA or epsilon C in the RNP to occur and permits one to estimate the average distance between Trp (presumably Trp 52)o and the RNA base binding region in the virus to be 17 to 20 A.
摘要
已开发出一种用于测定聚（A，εA）和聚（C，εC）共聚物的修饰度和摩尔消光系数的分光光度法。研究了共聚物的一些吸收和荧光参数对修饰度的依赖性。共聚物中修饰残基的分布不同于随机分布，并且取决于修饰条件。已研究了烟草花叶病毒（TMV）蛋白与共聚物之间的相互作用。该蛋白与低修饰度或中等修饰度的聚（A，εA）相互作用，而对聚（εA）无活性。相反，向多核苷酸中引入εC促进了聚（C，εC）与TMV蛋白之间的复合物形成。对荧光发射和激发光谱的分析表明，在核糖核蛋白（RNP）中发生了从色氨酸到εA或εC的能量转移，并使人们能够估计色氨酸（可能是色氨酸52）与病毒中RNA碱基结合区域之间的平均距离为17至20埃。
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