[悬浮吸附法在虫媒病毒研究中的应用]
[Use of suspension adsorption methods for studying arboviruses].
作者信息
Kokorev V S, Gaĭdamovich S Ia
出版信息
Vopr Virusol. 1977 Jul-Aug(4):488-92.
Hemagglutinating antigens of Western equine encephalomyelitis (WEE) virus and 5 strains of tick-borne encephalitis (TBE) virus were charcaterized by their capacity to adsorb on inorganic (kaolin, bentonite, barium sulphate) and organic (chick embryo fibroblasts) adsorbents. The adsorption capacity of TBE virus was more marked than that of WEE. The TBE virus strains 4072 and 1524 avid in serological tests adsorbed better on inorganic adsorbents than nonavid strains No. 80 and 1509. In contrast, nonavid strains interacted better with biologic adsorbents. The adsorption capacity of tissue culture antigens was superior to that of brain hemagglutinating antigens. By means of adsorption on kaolin and chick embryo fibroblasts hemagglutinating and nonhemagglutinating infections particles of WEE and TBE viruses which also differed in some biological properties were separated.
通过西部马脑炎（WEE）病毒和5株蜱传脑炎（TBE）病毒的血凝抗原吸附无机（高岭土、膨润土、硫酸钡）和有机（鸡胚成纤维细胞）吸附剂的能力对其进行了表征。TBE病毒的吸附能力比WEE病毒更为显著。在血清学试验中呈亲和性的TBE病毒株4072和1524比非亲和性株80和1509在无机吸附剂上的吸附效果更好。相反，非亲和性株与生物吸附剂的相互作用更好。组织培养抗原的吸附能力优于脑血凝抗原。通过高岭土和鸡胚成纤维细胞吸附，分离出了在某些生物学特性上也存在差异的WEE和TBE病毒的血凝感染性颗粒和非血凝感染性颗粒。
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