Dimitrova S, Labreva L
Vutr Boles. 1981;20(1):91-6.
The data from the intra-laboratory qualitative control art analyzed--accuracy and reproducibility of the results from the determinations of pH, pCO2 and pO2. Reproducibility is followed day by day and the accuracy of the results from the determinations of pH, pCO2, and pO2. The determinations were performed as follows: pH--by micro-electrode, pCO2--by equilibrium and by selective electrode, pO2--by electrode, Clark type with apparatuses VME 22, VME 32 and automatic analyser for blood-gas analysis ABL 2 (Radiometer). Acidbasol and Versatol Acid-Base were used as control materials in the three ranges: normal, acid and alkaline. Reproducibility of the results as regards time, presented by variation coefficient (VC), followed up for a period of 9 months by the control materials Acidbasol with ABL 2 apparatus proved to be very good in the three ranges tested for pH and pCO2. Results dispersion is higher with pO2 determinations, even higher with the low values. The accuracy of the results from pH and pCO2, determinations for the same period was within the allowable limits. A very good reproducibility of pH and pCO2 was indicated by the reproducibility data in time of the results from pH and pCO2 determinations from one year with the three apparatuses, making use of the control serum Versatol Acid-Base for the three ranges. Better variation coefficients by equilibrium method were established for the higher values of pCO2 with its automatic determination. High reliability of the investigation methods was established be the results from the systematic qualitative control of acid-base and blood-gas parameters.
对实验室内定性控制数据进行了分析——pH、pCO₂和pO₂测定结果的准确性和可重复性。逐日跟踪可重复性以及pH、pCO₂和pO₂测定结果的准确性。测定方法如下:pH——采用微电极法,pCO₂——采用平衡法和选择性电极法,pO₂——采用电极法,使用VME 22、VME 32型仪器以及ABL 2型血气自动分析仪(Radiometer公司)。在正常、酸性和碱性三个范围内,使用Acidbasol和Versatol酸碱作为对照物质。用ABL 2型仪器,通过对照物质Acidbasol对结果在时间方面的可重复性(以变异系数(VC)表示)进行了为期9个月的跟踪,结果表明在测试的pH和pCO₂的三个范围内非常好。pO₂测定结果的离散度更高,低值时更高。同期pH和pCO₂测定结果的准确性在允许范围内。利用对照血清Versatol酸碱在三个范围内对三种仪器pH和pCO₂测定结果在时间方面的可重复性数据表明,pH和pCO₂具有非常好的可重复性。对于较高值的pCO₂自动测定,平衡法建立了更好的变异系数。酸碱和血气参数的系统定性控制结果确立了研究方法的高可靠性。