Terreros D A, Tarr M, Grantham J J
Am J Physiol. 1981 Jul;241(1):F61-8. doi: 10.1152/ajprenal.1981.241.1.F61.
Isolated fragments of renal tubules were prepared by treating rabbit kidneys removed from animals on a normal diet with proteolytic enzymes. The intracellular potential of tubule cells was measured either by impaling cells across the luminal membrane at the everted end of the tubule or by impaling cells across the basolateral membrane along the shaft of the tubule. The mean intracellular potential recorded from 163 cells at the everted ends of proximal tubules was -49 +/- 12 mV (SD) compared with a mean value of -29 +/- 9 mV (SD) recorded from 199 cells along the shaft of the tubules. In papillary collecting ducts there was no significant difference between the intracellular potential of cells at the everted end of the tubules compared with those of cells along the shaft of the tubule. However, two distinctly different types of intracellular potentials were recorded from collecting duct cells regardless of the location of the cells: 93 cell impalements gave a mean intracellular potential of -15 +/- 8 mV (SD), whereas 135 cell impalements gave a mean potential of +24 +/- 12 mV (SD). The data presented in this paper demonstrate that intracellular potentials can be recorded from isolated renal tubule fragments of papillary collecting ducts and proximal convoluted tubules.
通过用蛋白水解酶处理取自正常饮食动物的兔肾来制备分离的肾小管片段。通过在肾小管外翻端穿过管腔膜刺入细胞，或沿着肾小管轴穿过基底外侧膜刺入细胞来测量肾小管细胞的细胞内电位。从近端肾小管外翻端的163个细胞记录的平均细胞内电位为-49±12mV（标准差），而从沿着肾小管轴的199个细胞记录的平均值为-29±9mV（标准差）。在乳头集合管中，与沿着肾小管轴的细胞相比，肾小管外翻端细胞的细胞内电位没有显著差异。然而，无论细胞位置如何，从集合管细胞记录到两种明显不同类型的细胞内电位：93次细胞刺入得到的平均细胞内电位为-15±8mV（标准差），而135次细胞刺入得到的平均电位为+24±12mV（标准差）。本文给出的数据表明，可以从乳头集合管和近端曲管的分离肾小管片段记录细胞内电位。
